Murine models: Home Office Personal Licence IF9D75154 - Categories A, B and C - animal handling; breeding; injections; anesthesia; surgical procedures; autopsy; microsurgery;
MCAo model; Cannulation of salivary glands; perfusions; blood and tissue collection.
Cells: In vitro culture of, both human and murine, lymphocytes and macrophages; PBMCs isolation; enzymatic tissue digestion for single cell suspensions.
Histology: Preparation and sectioning of frozen and paraffin embedded sections; immunohistochemistry; immunofluorescence; in situ zymography.
Biochemistry/Genetics: Isolation and analysis of DNA, RNA and protein; electrophoresis; blotting techniques; immunoprecipitation; cell fractionation; enzyme-linked
immunosorbent assays).
Laboratory/Research instruments: Seahorse; BOND max; spectrophotometer; autoanalyzer; pH meter; centrifuges; incubators; dark room equipment; precision balance; microtome and
cryostat; X-ray equipment.
Other skills or relevant experience:Science Illustrator; Data processing; statistical analysis;
programming skills; database skills; design, conduct and interpret scientific research; apply a scientific approach to problems; communicate findings using models, charts and graphs;
communicate new research findings to lay audiences; communicate biological research findings using scientific writing.
